Maganin ƙwayoyin cuta masu tasowa ta amfani da plasma mai arzikin platelet (PRP) na iya taka rawar taimako a cikin tsare-tsaren jiyya na farfadowa daban-daban.Akwai buƙatun da ba a cika duniya ba don dabarun gyara nama don kula da marasa lafiya da ke da ƙwayoyin tsoka (MSK) da cututtukan kashin baya, osteoarthritis (OA) da hadaddun raɗaɗi da raunin rauni.Maganin PRP ya dogara ne akan gaskiyar cewa ƙwayar platelet girma (PGF) tana goyan bayan raunin rauni da gyaran gyare-gyare (ƙumburi, haɓakawa da sake gyarawa).An kimanta nau'ikan nau'ikan nau'ikan PRP daban-daban daga nazarin ɗan adam, in vitro da dabba.Duk da haka, shawarwarin in vitro da nazarin dabba yawanci suna haifar da sakamako daban-daban na asibiti, saboda yana da wuya a fassara sakamakon binciken da ba na asibiti ba da kuma shawarwarin hanyoyin cikin maganin asibiti na mutum.A cikin 'yan shekarun nan, an sami ci gaba wajen fahimtar manufar fasaha na PRP da kwayoyin halitta, kuma an gabatar da sababbin umarnin bincike da sababbin alamu.A cikin wannan bita, za mu tattauna sabon ci gaba a cikin shirye-shiryen da abun da ke ciki na PRP, ciki har da kashi na platelet, aikin leukocyte da kuma tsarin rigakafi na ciki da kuma daidaitawa, 5-hydroxytryptamine (5-HT) sakamako da jin zafi.Bugu da ƙari, mun tattauna tsarin PRP da ke da alaka da kumburi da angiogenesis yayin gyaran nama da farfadowa.A ƙarshe, za mu sake nazarin tasirin wasu magunguna akan ayyukan PRP.
Autologous platelet-rich plasma (PRP) shine sashin ruwa na gefen jini na autologous bayan jiyya, kuma adadin platelet ya fi na asali.An yi amfani da maganin PRP don alamomi daban-daban fiye da shekaru 30, wanda ya haifar da babbar sha'awa ga yiwuwar PRP mai cin gashin kansa a cikin maganin farfadowa.An gabatar da kalmar wakili na nazarin halittu kwanan nan don magance cututtukan musculoskeletal (MSK), kuma ya sami sakamako mai ban sha'awa a cikin ikon farfadowa na gaurayewar kwayoyin halitta na PRP.A halin yanzu, maganin PRP shine zaɓin magani mai dacewa tare da fa'idodin asibiti, kuma sakamakon haƙuri da aka ruwaito yana ƙarfafawa.Koyaya, rashin daidaituwa na sakamakon haƙuri da sabbin fahimta sun haifar da ƙalubale ga aiwatar da aikace-aikacen asibiti na PRP.Ɗaya daga cikin dalilan na iya zama lamba da bambancin tsarin PRP da PRP-type akan kasuwa.Waɗannan na'urori sun bambanta dangane da girman tarin PRP da tsarin shirye-shiryen, wanda ke haifar da halaye na musamman na PRP da wakilai na halitta.Bugu da ƙari, rashin daidaituwa game da daidaitattun tsarin shirye-shiryen PRP da cikakken rahoton kwayoyin halitta a cikin aikace-aikacen asibiti ya haifar da sakamakon rashin daidaituwa.An yi ƙoƙari da yawa don siffata da rarraba PRP ko samfuran da aka samu daga jini a aikace-aikacen magani na farfadowa.Bugu da ƙari, abubuwan da suka samo asali na platelet, irin su platelet lysates, an gabatar da su don binciken kwayoyin halitta da kuma in vitro.
Ɗaya daga cikin sharhi na farko akan PRP an buga shi a cikin 2006. Babban abin da ke mayar da hankali kan wannan bita shine aiki da yanayin aikin platelet, tasirin PRP akan kowane mataki na cascade na warkarwa, da kuma ainihin rawar da ake samu na ci gaban platelet. a cikin alamun PRP daban-daban.A farkon matakin bincike na PRP, babban abin sha'awar PRP ko PRP-gel shine wanzuwa da takamaiman ayyuka na abubuwan haɓakar platelet da yawa (PGF).
A cikin wannan takarda, za mu tattauna dalla-dalla game da sabon ci gaba na nau'ikan nau'ikan ƙwayoyin cuta na PRP daban-daban da masu karɓan ƙwayoyin sel na platelet da tasirin su akan ƙa'idodin tsarin rigakafi na asali da daidaitacce.Bugu da ƙari, za a yi magana dalla-dalla dalla-dalla rawar da ƙwayoyin mutum ɗaya waɗanda zasu iya kasancewa a cikin jiyya na PRP da tasirin su akan tsarin farfadowa na nama.Bugu da ƙari, sabon ci gaba na fahimtar ma'aikatan ilimin halitta na PRP, adadin platelet, takamaiman tasirin ƙwayoyin jini na musamman, da kuma tasirin PGF da kuma cytokines akan tasirin sinadirai na kwayoyin halitta na mesenchymal (MSCs), ciki har da PRP da ke niyya daban-daban. yanayin tantanin halitta da nama bayan fassarar siginar tantanin halitta da tasirin paracrine.Hakazalika, za mu tattauna tsarin PRP da ke da alaka da kumburi da angiogenesis yayin gyaran nama da farfadowa.A ƙarshe, za mu sake nazarin tasirin analgesic na PRP, tasirin wasu kwayoyi akan ayyukan PRP, da haɗin PRP da shirye-shiryen gyarawa.
Ka'idoji na asali na maganin ƙwayar cuta mai wadatar platelet na asibiti
Shirye-shiryen PRP suna ƙara shahara kuma ana amfani da su sosai a fannonin likitanci daban-daban.Mahimmin ka'idar kimiyya ta PRP magani shine cewa allurar da aka tattara ta platelet a wurin da aka ji rauni na iya fara gyaran nama, haɓakar sabbin kayan haɗin gwiwa da sake sake zagayawa na jini ta hanyar sakin abubuwa da yawa masu aiki na halitta (alallolin girma, cytokines, lysosomes) da sunadaran adhesion da ke da alhakin fara amsawar hemostatic cascade.Bugu da ƙari, sunadaran sunadaran plasma (misali fibrinogen, prothrombin, da fibronectin) suna kasancewa a cikin abubuwan da ba su da kyau a cikin plasma (PPPs).Mahimmancin PRP na iya tayar da sakin hyperphysiological na abubuwan haɓaka don fara warkar da rauni na yau da kullun da kuma hanzarta aiwatar da gyaran gyare-gyare na mummunan rauni.A duk matakai na tsarin gyaran gyare-gyare na nama, nau'o'in nau'o'in girma, cytokines da masu kula da aikin gida suna inganta yawancin ayyukan kwayoyin halitta ta hanyar tsarin endocrin, paracrine, autocrine da endocrin.Babban abũbuwan amfãni na PRP sun haɗa da amincinsa da fasaha na shirye-shiryen fasaha na kayan aikin kasuwanci na yanzu, wanda za'a iya amfani dashi don shirya kwayoyin halitta wanda za'a iya amfani dashi ko'ina.Mafi mahimmanci, idan aka kwatanta da na kowa corticosteroids, PRP samfuri ne mai sarrafa kansa ba tare da sanin illa ba.Duk da haka, babu wani takamaiman ƙa'ida akan tsari da abun da ke tattare da injectable PRP abun da ke ciki, kuma abun da ke ciki na PRP yana da manyan canje-canje a cikin platelets, farin jini (WBC) abun ciki, gurɓataccen ƙwayar jini (RBC), da kuma PGF maida hankali.
Kalmomin PRP da rarrabawa
Shekaru da yawa, haɓaka samfuran PRP da aka yi amfani da su don haɓaka gyare-gyaren nama da haɓakawa ya kasance wani muhimmin fagen bincike na halittu da kimiyyar ƙwayoyi.Cascade na warkar da nama ya haɗa da mahalarta da yawa, ciki har da platelets da abubuwan haɓakarsu da granules na cytokine, ƙwayoyin farin jini, fibrin matrix da sauran cytokines masu haɗin gwiwa da yawa.A cikin wannan tsari na cascade, wani hadadden tsari na coagulation zai faru, ciki har da kunna platelet da haɓakawa na gaba da α- Sakin abubuwan da ke cikin ƙwayoyin platelet, haɗuwa da fibrinogen (wanda aka saki ta platelets ko kyauta a cikin plasma) zuwa cibiyar sadarwar fibrin, da samuwar. na platelet embolism.
"Universal" PRP yana kwatanta farkon waraka
Da farko, ana kiran kalmar “platelet-rich plasma (PRP)” da ake amfani da ita wajen maganin ƙarin jini, kuma har yanzu ana amfani da ita a yau.Da farko, waɗannan samfuran PRP an yi amfani da su ne kawai azaman mannen nama na fibrin, yayin da platelets kawai ana amfani da su don tallafawa fibrin polymerization mai ƙarfi don haɓaka hatimin nama, maimakon azaman mai kara kuzari.Bayan haka, an ƙera fasahar PRP don yin kwaikwayi farawar waraka.Daga bisani, an taƙaita fasahar PRP ta hanyar iyawarta na gabatarwa da saki abubuwan haɓakawa a cikin ƙananan ƙananan gida.Wannan sha'awar isar da PGF sau da yawa yana ɓoye muhimmiyar rawar sauran abubuwan da ke cikin waɗannan abubuwan haɗin jini.Wannan sha'awar ta ƙara ƙaruwa saboda rashin bayanan kimiyya, imani na sufanci, sha'awar kasuwanci da rashin daidaituwa da rarrabuwa.
Ilimin halitta na PRP maida hankali ne mai rikitarwa kamar jini kansa, kuma yana iya zama mafi rikitarwa fiye da magungunan gargajiya.Samfuran PRP sune abubuwan rayuwa masu rai.Sakamakon aikace-aikacen PRP na asibiti ya dogara ne akan abubuwan da ke cikin jiki, na duniya da kuma daidaitawa na jinin mai haƙuri, ciki har da wasu sassa daban-daban na salula waɗanda zasu iya kasancewa a cikin samfurin PRP da ƙananan ƙananan ƙananan mai karɓa, wanda zai iya zama a cikin m ko na yau da kullum.
Takaitacciyar ƙa'idodin PRP masu ruɗani da tsarin rarrabuwa da aka gabatar
Shekaru da yawa, masu aiki, masana kimiyya da kamfanoni suna fama da rashin fahimta na farko da lahani na samfuran PRP da sharuɗɗan su.Wasu mawallafa sun bayyana PRP a matsayin platelet-kawai, yayin da wasu suka nuna cewa PRP kuma ya ƙunshi jajayen ƙwayoyin jini, fararen jini daban-daban, fibrin da sunadaran bioactive tare da karuwa mai yawa.Sabili da haka, an gabatar da nau'o'in kwayoyin halitta na PRP daban-daban a cikin aikin asibiti.Abin takaici ne cewa wallafe-wallafen yawanci ba su da cikakken bayani game da kwayoyin halitta.Rashin gazawar daidaitattun shirye-shiryen samfuri da haɓaka tsarin rarrabuwa na gaba ya haifar da amfani da adadi mai yawa na samfuran PRP waɗanda aka bayyana ta hanyoyi daban-daban da gajarta.Ba abin mamaki ba ne cewa canje-canje a cikin shirye-shiryen PRP suna haifar da rashin daidaituwa ga sakamakon haƙuri.
Kingsley ya fara amfani da kalmar “platelet-rich plasma” a cikin 1954. Shekaru da yawa bayan haka, Ehrenfest et al.An gabatar da tsarin rarrabuwa na farko dangane da manyan masu canji guda uku (platelet, leukocyte da abun ciki na fibrin), kuma yawancin samfuran PRP sun kasu kashi huɗu: P-PRP, LR-PRP, fibrin mai arzikin platelet (P-PRF) da leukocytes. mai arziki PRF (L-PRF).Ana shirya waɗannan samfuran ta cikakken tsarin rufaffiyar atomatik ko yarjejeniya ta hannu.A halin yanzu, Everts et al.An jaddada muhimmancin ambaton fararen jini a cikin shirye-shiryen PRP.Suna kuma ba da shawarar amfani da kalmomin da suka dace don nuna nau'ikan shirye-shiryen PRP marasa aiki ko kunnawa da gel ɗin platelet.
Delong et al.ya ba da shawarar tsarin rarrabuwa na PRP da ake kira platelets, da aka kunna farin jini (PAW) dangane da cikakken adadin platelet, gami da jeri na tattara platelet huɗu.Sauran sigogi sun haɗa da amfani da masu kunnawa platelet da kasancewar ko rashin farin sel (watau neutrophils).Mishra et al.An gabatar da tsarin rarraba irin wannan.Bayan 'yan shekaru baya, Mautner da abokan aikinsa sun bayyana wani tsari mai zurfi da cikakken tsarin rarrabawa (PLRA).Marubucin ya tabbatar da cewa yana da mahimmanci don bayyana cikakken adadin platelet, abun ciki na farin jini (tabbatacce ko korau), adadin neutrophil, RBC (tabbatacce ko korau) da kuma ko ana amfani da kunnawa waje.A cikin 2016, Magalon et al.An buga rarrabuwar DEPA dangane da adadin allurar platelet, ingantaccen samarwa, tsabtar PRP da aka samu da aiwatar da kunnawa.Daga baya, Lana da abokan aikinta sun gabatar da tsarin rarraba MARSPILL, suna mai da hankali kan sel masu mononuclear na gefe.Kwanan nan, Kwamitin Ma'auni na Kimiyya ya ba da shawarar yin amfani da tsarin rarrabawa na Ƙungiyar Ƙasa ta Duniya don Thrombosis da Hemostasis, wanda ya dogara ne akan jerin shawarwarin yarjejeniya don daidaita amfani da samfurori na platelet a cikin aikace-aikacen magani na farfadowa, ciki har da daskararre da narke samfurori.
Dangane da tsarin rarraba PRP da masu aiki da masu bincike daban-daban suka gabatar, yunƙurin da ba su yi nasara ba don daidaitawa da samarwa, ma'anar da ma'anar PRP da likitocin likitoci za su yi amfani da su na iya zana kyakkyawan ƙarshe, wanda mai yiwuwa ba zai faru a cikin 'yan shekaru masu zuwa Bugu da ƙari. , Fasahar samfuran PRP na asibiti na ci gaba da haɓakawa, kuma bayanan kimiyya sun nuna cewa ana buƙatar shirye-shiryen PRP daban-daban don magance cututtuka daban-daban a ƙarƙashin takamaiman yanayi.Sabili da haka, muna sa ran cewa sigogi da masu canji na samar da PRP mai kyau za su ci gaba da girma a nan gaba.
Hanyar shirye-shiryen PRP tana ci gaba
Dangane da kalmomin PRP da bayanin samfur, ana fitar da tsarin rarrabuwa da yawa don ƙirar PRP daban-daban.Abin baƙin cikin shine, babu wata yarjejeniya akan cikakken tsarin rarrabuwa na PRP ko duk wani jini da samfuran jini na autologous.Da kyau, tsarin rarrabuwa ya kamata ya kula da halaye daban-daban na PRP, ma'anoni da ƙididdiga masu dacewa da suka danganci yanke shawara na jiyya na marasa lafiya da takamaiman cututtuka.A halin yanzu, aikace-aikacen orthopedic sun raba PRP zuwa nau'i uku: fibrin mai-arziƙin platelet (P-PRF), leukocyte-rich PRP (LR-PRP) da leukocytes-rauni PRP (LP-PRP).Kodayake ya fi takamaiman ma'anar samfurin PRP na gabaɗaya, LR-PRP da nau'ikan LP-PRP a fili ba su da takamaiman takamaiman abun cikin farin jini.Saboda tsarin garkuwar jiki da rundunar tsaro, fararen jini sun yi tasiri sosai ga ilimin halitta na cututtukan nama.Sabili da haka, ma'aikatan ilimin halitta na PRP waɗanda ke ɗauke da takamaiman ƙwayoyin jini na jini na iya haɓaka ƙa'idodin rigakafi da gyaran nama da haɓakawa sosai.Musamman ma, lymphocytes suna da yawa a cikin PRP, suna samar da nau'in haɓakar insulin-kamar kuma suna tallafawa gyaran nama.
Monocytes da macrophages suna taka muhimmiyar rawa a cikin tsarin tsarin rigakafi da tsarin gyaran nama.Muhimmancin neutrophils a cikin PRP ba a sani ba.An ƙaddara LP-PRP a matsayin shiri na farko na PRP ta hanyar kimantawa na yau da kullum don cimma sakamako mai kyau na jiyya na haɗin gwiwa OA.Duk da haka, Lana et al.Yin amfani da LP-PRP a cikin maganin OA na gwiwa yana adawa, wanda ke nuna cewa takamaiman ƙwayoyin jini na jini suna taka muhimmiyar rawa a cikin tsarin kumburi kafin farfadowa na nama, saboda suna sakin kwayoyin pro-inflammatory da anti-inflammatory.Sun gano cewa haɗuwa da neutrophils da platelets da aka kunna sun sami sakamako mai kyau fiye da mummunan tasiri akan gyaran nama.Har ila yau, sun nuna cewa filastik na monocytes yana da mahimmanci ga aikin rashin kumburi da gyaran gyare-gyare a gyaran nama.
Rahoton shirin shiri na PRP a cikin bincike na asibiti yana da rashin daidaituwa sosai.Yawancin binciken da aka buga ba su ba da shawarar hanyar shirye-shiryen PRP da ake buƙata don maimaita tsarin ba.Babu wata cikakkiyar yarjejeniya tsakanin alamun jiyya, don haka yana da wuya a kwatanta samfuran PRP da sakamakon maganin su.A mafi yawan lokuta da aka ruwaito, ana rarraba maganin tattarawar platelet a ƙarƙashin kalmar "PRP", har ma don nunin asibiti iri ɗaya.Ga wasu wuraren kiwon lafiya (irin su OA da tendinosis), an sami ci gaba wajen fahimtar canje-canjen shirye-shiryen PRP, hanyoyin bayarwa, aikin platelet da sauran abubuwan PRP waɗanda ke shafar gyaran nama da farfadowa na nama.Duk da haka, ana buƙatar ƙarin bincike don cimma matsaya kan ma'anar PRP da ke da alaƙa da ma'aikatan ilimin halitta na PRP don cikawa da aminci ga wasu cututtuka da cututtuka.
Matsayin tsarin rarraba PRP
Amfani da autologous PRP biotherapy yana damuwa da nau'in shirye-shiryen PRP, rashin daidaituwa suna da rashin daidaituwa na jagororin tushen shaida (wato, akwai hanyoyin shirye-shiryen da yawa don samar da vials na asibiti).Ana iya annabta cewa cikakken abun ciki na PRP, tsabta da halayen halittu na PRP da samfuran da ke da alaƙa sun bambanta sosai, kuma suna shafar tasirin ilimin halitta da sakamakon gwaji na asibiti.Zaɓin na'urar shiri na PRP yana gabatar da maɓalli na farko.A cikin maganin farfadowa na asibiti, masu aiki zasu iya amfani da kayan aikin shirye-shiryen PRP guda biyu da hanyoyi.Shiri yana amfani da daidaitaccen mai raba kwayar jini, wanda ke aiki akan cikakken jinin da aka tattara da kansa.Wannan hanyar tana amfani da drum mai ci gaba da gudana ko fasahar rabuwar diski da matakan centrifuge mai wuya da taushi.Yawancin waɗannan na'urori ana amfani da su wajen tiyata.Wata hanyar ita ce yin amfani da fasahar centrifugal mai nauyi da kayan aiki.Babban G-force centrifugation ana amfani da shi don raba rawaya Layer na ESR daga naúrar jini mai dauke da platelets da fararen jini.Waɗannan na'urorin tattarawa sun fi masu raba ƙwayoyin jini ƙanana kuma ana iya amfani da su a gefen gado.Bambance-bambancen ģ - Ƙarfi da lokacin ƙaddamarwa yana haifar da bambance-bambance masu mahimmanci a cikin yawan amfanin ƙasa, maida hankali, tsabta, iyawa, da kuma kunna yanayin da keɓaɓɓen platelets.Yawancin nau'ikan kayan shirye-shiryen PRP na kasuwanci za a iya amfani da su a cikin rukuni na ƙarshe, yana haifar da ƙarin canje-canje a cikin abun ciki na samfur.
Rashin daidaituwa akan hanyar shirye-shiryen da kuma tabbatar da PRP ya ci gaba da haifar da rashin daidaituwa na maganin PRP, kuma akwai babban bambance-bambance a cikin shirye-shiryen PRP, samfurin samfurin da sakamakon asibiti.An tabbatar da kayan aikin PRP na kasuwanci na yanzu kuma an yi rajista bisa ga ƙayyadaddun ƙira na masu sana'a, wanda ke warware sauye-sauye daban-daban a cikin kayan aikin PRP na yanzu.
Fahimtar adadin platelet a cikin vitro da in vivo
Tasirin warkewa na PRP da sauran abubuwan da ke tattare da platelet sun samo asali ne daga sakin abubuwa daban-daban da ke cikin gyaran nama da sabuntawa.Bayan kunna platelets, platelets za su samar da thrombus na platelet, wanda zai zama matrix na wucin gadi na wucin gadi don inganta yaduwar kwayar halitta da bambanta.Sabili da haka, yana da kyau a ɗauka cewa mafi girman adadin platelet zai haifar da mafi girma a cikin gida na abubuwan bioactive na platelet.Duk da haka, alaƙar da ke tsakanin kashi da tattarawar platelets da ƙaddamar da ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta na iya zama mai mahimmanci, saboda akwai bambance-bambance masu mahimmanci a cikin ƙididdiga na platelet tsakanin kowane marasa lafiya, kuma akwai bambance-bambance tsakanin hanyoyin shirye-shiryen PRP.Hakazalika, abubuwan haɓakar platelet da yawa waɗanda ke cikin tsarin gyaran nama suna cikin ɓangaren plasma na PRP (misali, haɓakar haɓakar hanta da haɓakar insulin-kamar factor factor 1).Don haka, mafi girman adadin platelet ba zai shafi yuwuwar gyara waɗannan abubuwan haɓaka ba.
Binciken in vitro PRP ya shahara sosai saboda sigogi daban-daban a cikin waɗannan karatun ana iya sarrafa su daidai kuma ana iya samun sakamakon da sauri.Yawancin karatu sun nuna cewa sel suna amsawa ga PRP ta hanyar dogaro da kashi.Nguyen da Pham sun nuna cewa babban taro na GF ba lallai ba ne ya dace da aiwatar da kuzarin tantanin halitta, wanda zai iya zama mara amfani.Wasu nazarin in vitro sun nuna cewa babban taro na PGF na iya haifar da illa.Dalili ɗaya na iya zama iyakataccen adadin masu karɓan membrane.Sabili da haka, da zarar matakin PGF ya yi yawa idan aka kwatanta da masu karɓa na samuwa, za su sami mummunan tasiri akan aikin salula.
Muhimmancin bayanan tattarawar platelet a cikin vitro
Kodayake binciken in vitro yana da fa'idodi da yawa, amma yana da wasu rashin amfani.A cikin vitro, saboda ci gaba da hulɗar tsakanin nau'ikan tantanin halitta daban-daban a cikin kowane nau'in nama saboda tsarin nama da nama na salula, yana da wuya a yi maimaita a cikin vitro a cikin yanayin al'ada guda biyu mai girma.Yawan tantanin halitta wanda zai iya shafar hanyar siginar tantanin halitta yawanci kasa da 1% na yanayin nama.Nama na al'ada mai girma biyu yana hana sel fallasa zuwa matrix extracellular (ECM).Bugu da ƙari, fasaha na al'ada na yau da kullum zai haifar da tarawar sharar tantanin halitta da ci gaba da cin abinci mai gina jiki.Sabili da haka, al'adun in vitro ya bambanta da kowane yanayin da ya dace, samar da iskar oxygen na nama ko musayar musayar al'ada kwatsam, kuma an buga sakamakon rikice-rikice, kwatanta tasirin asibiti na PRP tare da nazarin in vitro na takamaiman sel, nau'in nama da platelet. maida hankali.Graziani da sauransu.An gano cewa a cikin vitro, mafi girman tasiri akan yaduwar osteoblasts da fibroblasts an samu a cikin PRP platelet maida hankali sau 2.5 fiye da darajar asali.Sabanin haka, bayanan asibiti da Park da abokan aiki suka bayar sun nuna cewa bayan haɗin gwiwa na kashin baya, matakin PRP platelet yana buƙatar ƙarawa fiye da sau 5 fiye da tushe don haifar da sakamako mai kyau.Hakanan an ba da rahoton irin wannan sakamako masu cin karo da juna tsakanin bayanan yaɗuwar jijiya a cikin vitro da sakamakon asibiti.
(Ana sake buga abubuwan da ke cikin wannan labarin, kuma ba mu bayar da garantin bayyananne ko bayyananniyar garanti don daidaito, amintacce ko cikar abubuwan da ke cikin wannan labarin ba, kuma ba mu da alhakin ra'ayoyin wannan labarin, da fatan za a fahimta.)
Lokacin aikawa: Maris-01-2023